Wrinkle-free tissue sections

Preparation of Tissue Sections

A simple reproducible protocol for detecting multiple copy human genes and viral DNA in routine formalin fixed paraffin embedded tonsil and brain, by in situ hybridisation with biotinylated probes, is described. The protocol consists of digestion of formalin fixed paraffin sections, with 0 4% pepsin in 0 01 M hydrochloric acid for one hour at 37°C, followed by hybridisation with biotinylated pr...

Mixed Agglutination with Tissue Sections

The mixed agglutination procedure was applied to tissue sections. Microtome sections of bovine tissue were placed on coverglasses, fixed by acetone or formalin, and incubated with various dilutions of rabbit antisera. The binding of antibodies to the tissue sections was detected by the addition of an indicator system composed of sheep erythrocytes sensitized by subagglutinating doses of the cor...

A simple and sensitive antigen retrieval method for free-floating and slide-mounted tissue sections.

The masking of antigens by aldehyde-containing fixatives or by paraffin embedding procedures is a problem for immunohistochemical studies. Enzymatic digestion, formic acid treatment, microwave heating and autoclave heating have been used to deal with this problem, with microwave heating-based antigen retrieval having become widely used as the method of choice. Microwave heating, however, has th...

Notes on the electron microscopy of tissue sections; neoplastic tissue.

In the s tudy of tissue sections with the electron microscope hundreds of micrographs have impressed us with some aspects characteristic of the over-all picture of cellular structure. We have observed the cells in our sections to exhibit such distinctive traits that we feel justified in listing some of these as indicative of normal tissue and some as pointing to neoplastic phenomena, regardless...

Non-nuclear staining of thick tissue sections.